Any one of those was probably enough for Trump to lose the debate. However if I was Kellyanne Conway (which thankfully I am not
), the thing that would concern me most would be how easily "baited" Trump was. He cannot help himself and will respond to any provocation, even if it is obviously a ploy. Because of this, he gets off his talking points too easily (who cares about his 10 year old being good at computers, as opposed to NOT pointing out that his opponent privatized government cyber-communications). Because of this, he will make more gaffes in the coming debates, and his "team" can't even counsel him.
So in essence, the main thing that hurts him is his "temperament". What was his trademark to success until now will become his downfall.